Testing for environmental toxins has never been simpler!
With DBS, just a small prick of your finger is all it takes.
Just three steps to an answer: Collect, Analyze and Report
Our approach requires less blood and improves test accuracy
How do I use DBS to collect a sample?
Order individual test or a test panel through physician. A DBS card with test-specific isotope standards impregnated in it arrives at your home or at your doctor’s office.
The chain of custody is established when the envelope is sealed.
What is Dried Blood Spot Analysis?
Dried Blood Spot analysis (DBS) is a form of bio-sampling where blood samples, typically from a finger or heel stick, are blotted and dried on filter paper for shipment, analysis, and storage.
Dried blood spot analysis has many advantages over clinical blood draws including:
- No need for cold storage
- No phlebotomist required
- Facilitates pediatric studies
The Advantages of DBS vs. a Typical Blood Draw
How Does Direct Isotope Dilution Mass Spectrometry Provide More Accurate Results?
Direct Isotope Dilution Mass Spectrometry (D-IDMS) is a state-of-the-art analytical technique that dramatically increases the accuracy, precision, and reproducibility of analytical instrumentation used in clinical analysis, and has the unique ability to track and measure changes in samples from the moment of collection to the instant of analysis.
D-IDMS has been proven in the chemical, energy, and environmental fields for many years, however has only recently been applied to biological and clinical samples. It is regarded by the National Institute of Standards & Technology (NIST) as a “definitive method” because it is a method of proven high accuracy. The accuracy is afforded by adding a known amount of a non-radioactive or so-called “stable” chemical isotope to the sample.
Suppose a blood sample contains many things, including the elements zinc, (Zn) iron (Fe), and lead (Pb). We want to know how much lead (Pb) is present, but how do you count all of the lead in the blood? To further confuse things, lead has four stable isotopes 204Pb, 206Pb, 207Pb and 208Pb. In nature, the abundance of these isotopic forms of lead are 1.4%, 24.1%, 22.1%, and 52.4% respectively.
How is direct isotope dilution mass spectrometry used to determine how much and which type of lead is in your blood? A known quantity of our lead isotope is combined with the sample. We add some 207Pb and 208Pb in an exact ratio that we know. We can now analyze the total amount of lead, and the amount of each isotope. The addition of the isotope allows us to measure if there was a contamination, or in the case of biomarkers, a change in the sample from point of collection to point of analysis, by ensuring the proper amount and ratios are accounted for.
In summary, direct Isotope dilution mass spectrometry spikes a stable isotope of the chemical whose concentration (amount) in the sample is unknown. By measuring the masses of the isotopes added and the total of the mass of the known spike and unknown sample using mass spectrometry, a ratio of the spiked isotope to the unknown and total amounts can be determined and a more accurate result reported.